PREGVET

Cow Pregnancy Test Kit

IMPROVES EFFICIENCY LOSSES
ONLY METHOD CURRENTLY AVAILABLE TO DETECT PREGNANCY AS EARLY AS 17TH POST INSEMINATION DAY

PREGOVET Test

  • A female sex hormone Progesterone commonly found in pregnancy, maintain the entire gestation period of a Cow.
  • Progesterone is produced by the corpus luteum (CL) found in ovary, forms just after ovulation (release of egg from ovary). High progesterone levels indicates functional corpus luteum.
  • The PregOvet Test were optimized for the level of Progesterone hormone in milk between 20-25th post insemination days ( The level are <2.5ng/ml in Negative cases, and > 20ng/ml in Positive cases).
  • If a cow is not pregnant, the CL regress and progesterone levels decline to low levels till the cow comes into the next heat. But if a cow is pregnant, the CL continues to function and progesterone levels remain high throughout the gestation period.

PREGVET Test to Confirm Estrus

It has been seen that 10 to 20 percent of the cows inseminated were not in actual estrus at the time of  insemination and so there is no chance of  conceiving. If there is any suspect whether the cow is in estrus or not, a single milk sample test with Pregvet would indicate that cow is in estrus or not. High progesterone (white color) level would indicate that the cow is not in estrus and would not conceive if inseminated. Low progesterone (Bright blue color) level would suggest that the cow might be in estrus or nearer to it.

Progesterone Estimation Is The Only Test That Detects Pregnancy Status In Animals As Early As 20th Post Insemination Day.

PREGVET  Test Detect Early Pregnancy: To use the Pregvet test as a pregnancy indicator the milk sample must be collected between 20 to 24 days after  insemination Low progesterone (Bright Blue color) would indicate that the cow is not pregnant and high progesterone (White Color) would indicate that the cow has a functional corpus luteum and might be pregnant.

The milk progesterone test is one of the most important management aid, which can help to improve reproductive efficiency. For the successful reproduction one has to detect estrus correctly.

Pregvet accurately detect The Level of Progesterone in the Milk. Because A Cow with High Progesterone can not be OPEN (Estrus) and a Cow with Low Progesterone cannot be Pregnant.

Test Procedure is simple and easy, can be used as cow side test

  • Profitable Dairy Depend Upon: Maintaining the Inter-Calving period within 13 months.
  • Only 35-38% of all inseminations goes to full term.
  • Nearly 20% of all animals have irregular estrus cycle.
  • Presently pregnancy is confirmed only after 90 days by palpation / or   by expensive scanning after 35th post insemination days.

Perform test only between 17-21 post insemination days, Testing on other days may lead to wrong result due to variation of progesterone levels in milk. Perform subsequent test in positive case in between 45-50 post insemination days to confirm pregnancy.

In negative result follow the animal till they return to heat on 22nd post insemination day and reinseminate the animal. If they fail to return to heat by 23rd post insemination day hormone injection are recommended to make the animal return to heat within 48 hours and then they are inseminated, thereby inter-calving period is saved.

If allowed to return to heat by itself which may extend anywhere between 30-45 post insemination day which will result in 2nd delivery to extend beyond 13 months

THE ABOVE PROCEDURE IS TO EXECUTED ONLY UNDER THE GUIDANCE OF A VETERINARY PROFESSION

Early detection of pregnancy becomes mandatory for a profitable dairy farm

Inter-calving period should be maintain within 13 months

The milk yield will be maximum for first 6 months after calf births and then gradually reduce if 13 months post delivery is taken for calculating profit/loss, dairy accounts will reflect profit but beyond 13 months the result will reveal loss.

Reproductive Cycle : Nearly 20% of animals have irregular estrus cycle. They returns to heat after insemination if they are not pregnant anywhere between 35th – 45th post insemination days. This will extend inter calving period beyond 13 months resulting in loss.

Test Procedure

Remove Tubes from the cover. Tap the TUBE to make settle the fluid at bottom. Open Cap insert tube into the tube stand.

  • STEP-1 : Remove strip from cover
  • STEP-2 : Fill Milk in Sample Tube
  • STEP-3 : Add ONE drop of Milk Sample in TUBE NO-1 Mix Well
  • STEP-4 : Insert STRIP into TUBE NO-1. Membrane portion of Strip should be immersed
  • STEP-5 : After 5 minutes insert Strip into WASH TUBE for Half a Minute
  • STEP-6 : Insert Strip into TUBE NUMBER-2 with membrane portion immersed into liquid Incubate for 5 minutes
  • STEP-7 : After 5 Minutes insert strip into wash liquid for half a minute STEP-8 : Insert Strip into the TUBE NUMBER-3 with membrane portion immersed Read result after 7-10 minutes

IF TUBE NUMBER-3 DEVELOP DARK BLUE COLOR-LOW PROGESTERONE-LEVEL NEGATIVE FOR PREGNANCY

IF IT DEVELOP LIGHT COLOR- HIGH PROGESTERONE LEVEL- POSITIVE FOR PREGNANCY